Animated TV comedy Family Guy has taken another stab at California-based royal Prince Harry.

The hit cartoon’s second swipe at the Duke of Sussex in less than two years came in an episode called “Twains World”, which aired in the US last Thursday.

It shows characters Brian the talking Labrador and Stewie (both voiced by creator Seth MacFarlane) travelling back in time. As they chat, Brian puts Harry and his wife in a lineup with some of history’s worst events.

After Stewie warns Brian that they may be about to “change the course of history”, the dog says, “You always say that, but doesn’t history pretty much suck?”

He mentions Hitler and the Crusades before Stewie quips: “Prince Harry marrying Meghan Markle.”

At that point, the show cuts away to show a cartoon Harry watching from his couch.

“Again?” he complains, before turning toward the camera with his hands on his hips to say, “Oh, like all of your wives are so much better?”

The Duke and Duchess of Sussex are yet to make any public statement about the latest jab, which came on the same day it emerged the prince had recently travelled to Angola to walk in his late mother’s footsteps down a path in an active mine field.

The episode came less than two years after Family Guy‘s earlier mockery of Prince Harry and Meghan.

In that show, main character, Peter Griffin, is shown sitting in a bar with his friends as he mulls ways to retrieve money he is owed. Eventually, he says he’ll “go it alone”.

The show then cuts to Harry and Meghan, who are sunbathing beside a pool. A butler approaches the prince with a stack of envelopes.

“Sir, your millions from Netflix for … no one knows what,” he says.

Cartoon Harry tells him to “Put it with the rest of them”.

Then Meghan gets a notification on her phone.

“Babe, time to do our daily $250,000 sponsored Instagram post for [fast-food chain] Del Taco,” she says.

To which Harry replies: “I shouldn’t have left the made-up nonsense.”

It also follows a dig by another animated TV comedy, South Park, which infamously featured the Sussexes in a 22-minute episode titled “The Worldwide Privacy Tour”.

It showed Harry and Meghan – dubbed the “prince and princess of Canada” – being booed by the rest of the royal family because they had been “bashing the Canadian monarchy”.

The prince and his wife were also shown on a TV talk show, where they walked out chanting “we want privacy”. Both held signs, with hers saying “stop looking at us”, as they were booed by the audience.

South Park has also previously taken aim at Harry’s estranged brother, Prince William and his wife Kate. The Prince and Princess of Wales were the unlucky stars of a 2011 episode that mocked their royal wedding.